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criterion tgx precast protein gel  (Bio-Rad)


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    Structured Review

    Bio-Rad criterion tgx precast protein gel
    Criterion Tgx Precast Protein Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgx+precast+midi+protein+gel/10%25+Criterion+TGX+Precast+Midi+Protein+Gel/pmc13148909-279-6-11
    Average 96 stars, based on 173 article reviews
    criterion tgx precast protein gel - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: Titin's P-zone domains A164-167 are essential for thick filament structural arrangement.
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g. The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g. An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Article Title: Titin’s P-zone domains A164–167 are essential for thick filament structural arrangement
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g . The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g . An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Incubation:

    Article Title: Titin's P-zone domains A164-167 are essential for thick filament structural arrangement.
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g. The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g. An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Article Title: Titin’s P-zone domains A164–167 are essential for thick filament structural arrangement
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g . The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g . An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Centrifugation:

    Article Title: Titin's P-zone domains A164-167 are essential for thick filament structural arrangement.
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g. The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g. An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Article Title: Titin’s P-zone domains A164–167 are essential for thick filament structural arrangement
    Article Snippet: .. First, 10 μl SDS-PAGE buffer (4% SDS, 0.0625 M Tris-HCl, 10% glycerol, 0.2% bromophenol blue, 8 M urea, 5% β-mercaptoethanol) was added to each mini spin column, columns were incubated in a 95°C water both for 4 min, and elution into a fresh 2-ml tube (Eppendorf) was performed by centrifugation for 1 min at 1,000 × g . The second elution step was performed as above using 20 μl SDS–urea buffer and by centrifuging for 30 s at 10,000 × g . An entire eluate was loaded onto a Criterion 4–20% TGX Precast Midi Protein Gel (#5671095; Bio-Rad) and resolved by SDS-PAGE. ..

    Control:

    Article Title: Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal ENO1 antibody Abcam Cat# ab112994; RRID: AB_10858766 Rabbit monoclonal ENO1 antibody Abcam Cat#ab155102 Rabbit polyclonal Nucleolin antibody Abcam Cat#ab50279; RRID: AB_881762 Rabbit monoclonal Biotin antibody Abcam Cat#ab234284 Mouse monoclonal Biotin antibody Abcam Cat#ab201341; RRID: AB_2861249 Goat anti-mouse IgG H&L (HRP) Abcam Cat# ab6789; RRID: AB_955439 Goat anti-rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit polyclonal LARP1 antibody Bethyl Laboratories Cat#A302-088A; RRID: AB_1604275 Mouse monoclonal HA.11 epitope tag antibody Biolegend Cat#MMS-101P; RRID: AB_2314672 Normal Rabbit IgG Cell Signaling Cat#2729; RRID: AB_591709 Rabbit monoclonal SIRT2 antibody Cell Signaling Technology Cat#12650S; RRID: AB_2716762 Mouse monoclonal LC3 antibody Cosmo Bio Cat#cac-ctb-lc3-2-ic; RRID: AB_10707197 Rabbit Acetyl Lysine antibody Immunechem Cat#ICP0380; RRID: AB_2801477 Rabbit IgG isotype control Invitrogen Cat#10500C; RRID: AB_2532981 Mouse monoclonal anti-rabbit light chain antibody Millipore Cat#MAB201P; RRID: AB_827270 Goat polyclonal anti-mouse light chain antibody Millipore Cat#M AP200P; RRID: AB_805324 Mouse monoclonal ENO1 antibody Novus Cat# H00002023-M01; RRID: AB_463881 Mouse monoclonal ENO3 antibody Novus Cat#H00002027-M01; RRID: AB_509354 Rabbit polyclonal ENO1 antibody Proteintech Cat#11204-1-AP; RRID: AB_2099064 Rabbit polyclonal SLC3A2 antibody Santa Cruz Cat#sc-9160; RRID: AB_638288 Normal mouse IgG Santa Cruz Cat#sc-2025; RRID: AB_737182 Mouse monoclonal b-Actin antibody Sigma Cat#A5441; RRID: AB_476744 Mouse monoclonal FLAG antibody Sigma Cat#F3165; RRID: AB_259529 Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Mouse IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21202; RRID: AB_141607 Bacterial and virus strains E.coli BL21(DE3) CodonPlus-RIL Agilent Cat#: 230240 Chemicals, peptides, and recombinant proteins 1,4 Dithiothreitol (DTT) Biomol Cat#: 04010.10 40% Acrylamide/Bis Solution, 19:1 BioRad Cat#: 1610144 Protein Assay Dye Reagent Concentrate BioRad Cat#: 5000006 4–15% TGX Precast Midi Protein Gel, 18 well BioRad Cat#: 5671084 4–15% TGX Precast Midi Protein Gel, 12+2 well BioRad Cat#: 5671083 4–15% TGX Precast Midi Protein Gel, 26 well BioRad Cat#: 5671085 4–12%XTBis-Tris Precast Midi Protein Gel, 18 well BioRad Cat#: 3450123 (Continued on next page) Molecular Cell 82, 2666–2680.e1–e11, July 21, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trans-Blot Turbo Midi 0.2 mmNitrocellulose Transfer Packs BioRad Cat#: 1704159 SureBeads Protein G magnetic beads BioRad Cat#: 161-4023 Ammonium chloride (15N, 99%) Cambridge Isotope Laboratories Cat#: NLM-467 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat#: DLM-4 D-Glucose (U-13C6, 99%) Microbiological/ Pyrogen tested Cambridge Isotope Laboratories Cat#: CLM-1396-MPT-PK [g-32P] ATP, 6000 Ci/mmol, 10 mCi/ml Hartmann Cat#: SRP-501 TruSeq Stranded mRNA Library Preparation kit Illumina Cat#: 20020594 EmbryoMax ES Cell Qualified FBS, sterilefiltered, suitable for stem cell culture Merck Cat#: ES-009-B ESGRO Leukemia Inhibitory Factor (LIF) supplement Merck Cat#: ESG1106 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Merck Cat#: T9281 Anti-FLAG M2 Magnetic Beads Merck Cat#: M8823 Benzonase, 25 U/ mL Merck Millipore Cat#: 71206 Trichloroacetic acid Merck Millipore Cat#: 100807 Electrochemiluminescence (ECL) solution Merck Millipore Cat#: WBKLS0500 T4 Polynucleotide Kinase (PNK) New England BioLabs Cat#: M0201L Proteinase K New England BioLabs Cat#: P8107S HRV 3C protease Protein Purification Core Facility (EMBL) N/A Ni-NTA Agarose Qiagen Cat#: 30210 cOmplete Protease Inhibitor Cocktail Roche Cat#: 11697498001 DAPI Roche Cat#: 10236276001 Imidazole Roth Cat#: 3899.4 Gelatine Sigma Cat#: G1890 2-Mercaptoethanol Sigma Cat#: M6250 Isopropyl b-D-1thiogalactopyranoside (IPTG) Sigma Cat#: I5502 Adonitol, 99% Thermo Fisher Scientific Cat#: L03253 Methoxylamine hydrochloride, 25-30% aq. soln.

    Virus:

    Article Title: Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal ENO1 antibody Abcam Cat# ab112994; RRID: AB_10858766 Rabbit monoclonal ENO1 antibody Abcam Cat#ab155102 Rabbit polyclonal Nucleolin antibody Abcam Cat#ab50279; RRID: AB_881762 Rabbit monoclonal Biotin antibody Abcam Cat#ab234284 Mouse monoclonal Biotin antibody Abcam Cat#ab201341; RRID: AB_2861249 Goat anti-mouse IgG H&L (HRP) Abcam Cat# ab6789; RRID: AB_955439 Goat anti-rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit polyclonal LARP1 antibody Bethyl Laboratories Cat#A302-088A; RRID: AB_1604275 Mouse monoclonal HA.11 epitope tag antibody Biolegend Cat#MMS-101P; RRID: AB_2314672 Normal Rabbit IgG Cell Signaling Cat#2729; RRID: AB_591709 Rabbit monoclonal SIRT2 antibody Cell Signaling Technology Cat#12650S; RRID: AB_2716762 Mouse monoclonal LC3 antibody Cosmo Bio Cat#cac-ctb-lc3-2-ic; RRID: AB_10707197 Rabbit Acetyl Lysine antibody Immunechem Cat#ICP0380; RRID: AB_2801477 Rabbit IgG isotype control Invitrogen Cat#10500C; RRID: AB_2532981 Mouse monoclonal anti-rabbit light chain antibody Millipore Cat#MAB201P; RRID: AB_827270 Goat polyclonal anti-mouse light chain antibody Millipore Cat#M AP200P; RRID: AB_805324 Mouse monoclonal ENO1 antibody Novus Cat# H00002023-M01; RRID: AB_463881 Mouse monoclonal ENO3 antibody Novus Cat#H00002027-M01; RRID: AB_509354 Rabbit polyclonal ENO1 antibody Proteintech Cat#11204-1-AP; RRID: AB_2099064 Rabbit polyclonal SLC3A2 antibody Santa Cruz Cat#sc-9160; RRID: AB_638288 Normal mouse IgG Santa Cruz Cat#sc-2025; RRID: AB_737182 Mouse monoclonal b-Actin antibody Sigma Cat#A5441; RRID: AB_476744 Mouse monoclonal FLAG antibody Sigma Cat#F3165; RRID: AB_259529 Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Mouse IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21202; RRID: AB_141607 Bacterial and virus strains E.coli BL21(DE3) CodonPlus-RIL Agilent Cat#: 230240 Chemicals, peptides, and recombinant proteins 1,4 Dithiothreitol (DTT) Biomol Cat#: 04010.10 40% Acrylamide/Bis Solution, 19:1 BioRad Cat#: 1610144 Protein Assay Dye Reagent Concentrate BioRad Cat#: 5000006 4–15% TGX Precast Midi Protein Gel, 18 well BioRad Cat#: 5671084 4–15% TGX Precast Midi Protein Gel, 12+2 well BioRad Cat#: 5671083 4–15% TGX Precast Midi Protein Gel, 26 well BioRad Cat#: 5671085 4–12%XTBis-Tris Precast Midi Protein Gel, 18 well BioRad Cat#: 3450123 (Continued on next page) Molecular Cell 82, 2666–2680.e1–e11, July 21, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trans-Blot Turbo Midi 0.2 mmNitrocellulose Transfer Packs BioRad Cat#: 1704159 SureBeads Protein G magnetic beads BioRad Cat#: 161-4023 Ammonium chloride (15N, 99%) Cambridge Isotope Laboratories Cat#: NLM-467 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat#: DLM-4 D-Glucose (U-13C6, 99%) Microbiological/ Pyrogen tested Cambridge Isotope Laboratories Cat#: CLM-1396-MPT-PK [g-32P] ATP, 6000 Ci/mmol, 10 mCi/ml Hartmann Cat#: SRP-501 TruSeq Stranded mRNA Library Preparation kit Illumina Cat#: 20020594 EmbryoMax ES Cell Qualified FBS, sterilefiltered, suitable for stem cell culture Merck Cat#: ES-009-B ESGRO Leukemia Inhibitory Factor (LIF) supplement Merck Cat#: ESG1106 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Merck Cat#: T9281 Anti-FLAG M2 Magnetic Beads Merck Cat#: M8823 Benzonase, 25 U/ mL Merck Millipore Cat#: 71206 Trichloroacetic acid Merck Millipore Cat#: 100807 Electrochemiluminescence (ECL) solution Merck Millipore Cat#: WBKLS0500 T4 Polynucleotide Kinase (PNK) New England BioLabs Cat#: M0201L Proteinase K New England BioLabs Cat#: P8107S HRV 3C protease Protein Purification Core Facility (EMBL) N/A Ni-NTA Agarose Qiagen Cat#: 30210 cOmplete Protease Inhibitor Cocktail Roche Cat#: 11697498001 DAPI Roche Cat#: 10236276001 Imidazole Roth Cat#: 3899.4 Gelatine Sigma Cat#: G1890 2-Mercaptoethanol Sigma Cat#: M6250 Isopropyl b-D-1thiogalactopyranoside (IPTG) Sigma Cat#: I5502 Adonitol, 99% Thermo Fisher Scientific Cat#: L03253 Methoxylamine hydrochloride, 25-30% aq. soln.

    Recombinant:

    Article Title: Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal ENO1 antibody Abcam Cat# ab112994; RRID: AB_10858766 Rabbit monoclonal ENO1 antibody Abcam Cat#ab155102 Rabbit polyclonal Nucleolin antibody Abcam Cat#ab50279; RRID: AB_881762 Rabbit monoclonal Biotin antibody Abcam Cat#ab234284 Mouse monoclonal Biotin antibody Abcam Cat#ab201341; RRID: AB_2861249 Goat anti-mouse IgG H&L (HRP) Abcam Cat# ab6789; RRID: AB_955439 Goat anti-rabbit IgG H&L (HRP) Abcam Cat# ab97051; RRID: AB_10679369 Rabbit polyclonal LARP1 antibody Bethyl Laboratories Cat#A302-088A; RRID: AB_1604275 Mouse monoclonal HA.11 epitope tag antibody Biolegend Cat#MMS-101P; RRID: AB_2314672 Normal Rabbit IgG Cell Signaling Cat#2729; RRID: AB_591709 Rabbit monoclonal SIRT2 antibody Cell Signaling Technology Cat#12650S; RRID: AB_2716762 Mouse monoclonal LC3 antibody Cosmo Bio Cat#cac-ctb-lc3-2-ic; RRID: AB_10707197 Rabbit Acetyl Lysine antibody Immunechem Cat#ICP0380; RRID: AB_2801477 Rabbit IgG isotype control Invitrogen Cat#10500C; RRID: AB_2532981 Mouse monoclonal anti-rabbit light chain antibody Millipore Cat#MAB201P; RRID: AB_827270 Goat polyclonal anti-mouse light chain antibody Millipore Cat#M AP200P; RRID: AB_805324 Mouse monoclonal ENO1 antibody Novus Cat# H00002023-M01; RRID: AB_463881 Mouse monoclonal ENO3 antibody Novus Cat#H00002027-M01; RRID: AB_509354 Rabbit polyclonal ENO1 antibody Proteintech Cat#11204-1-AP; RRID: AB_2099064 Rabbit polyclonal SLC3A2 antibody Santa Cruz Cat#sc-9160; RRID: AB_638288 Normal mouse IgG Santa Cruz Cat#sc-2025; RRID: AB_737182 Mouse monoclonal b-Actin antibody Sigma Cat#A5441; RRID: AB_476744 Mouse monoclonal FLAG antibody Sigma Cat#F3165; RRID: AB_259529 Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Mouse IgG (H+L) Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21202; RRID: AB_141607 Bacterial and virus strains E.coli BL21(DE3) CodonPlus-RIL Agilent Cat#: 230240 Chemicals, peptides, and recombinant proteins 1,4 Dithiothreitol (DTT) Biomol Cat#: 04010.10 40% Acrylamide/Bis Solution, 19:1 BioRad Cat#: 1610144 Protein Assay Dye Reagent Concentrate BioRad Cat#: 5000006 4–15% TGX Precast Midi Protein Gel, 18 well BioRad Cat#: 5671084 4–15% TGX Precast Midi Protein Gel, 12+2 well BioRad Cat#: 5671083 4–15% TGX Precast Midi Protein Gel, 26 well BioRad Cat#: 5671085 4–12%XTBis-Tris Precast Midi Protein Gel, 18 well BioRad Cat#: 3450123 (Continued on next page) Molecular Cell 82, 2666–2680.e1–e11, July 21, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trans-Blot Turbo Midi 0.2 mmNitrocellulose Transfer Packs BioRad Cat#: 1704159 SureBeads Protein G magnetic beads BioRad Cat#: 161-4023 Ammonium chloride (15N, 99%) Cambridge Isotope Laboratories Cat#: NLM-467 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat#: DLM-4 D-Glucose (U-13C6, 99%) Microbiological/ Pyrogen tested Cambridge Isotope Laboratories Cat#: CLM-1396-MPT-PK [g-32P] ATP, 6000 Ci/mmol, 10 mCi/ml Hartmann Cat#: SRP-501 TruSeq Stranded mRNA Library Preparation kit Illumina Cat#: 20020594 EmbryoMax ES Cell Qualified FBS, sterilefiltered, suitable for stem cell culture Merck Cat#: ES-009-B ESGRO Leukemia Inhibitory Factor (LIF) supplement Merck Cat#: ESG1106 N,N,N0,N0-Tetramethylethylenediamine (TEMED) Merck Cat#: T9281 Anti-FLAG M2 Magnetic Beads Merck Cat#: M8823 Benzonase, 25 U/ mL Merck Millipore Cat#: 71206 Trichloroacetic acid Merck Millipore Cat#: 100807 Electrochemiluminescence (ECL) solution Merck Millipore Cat#: WBKLS0500 T4 Polynucleotide Kinase (PNK) New England BioLabs Cat#: M0201L Proteinase K New England BioLabs Cat#: P8107S HRV 3C protease Protein Purification Core Facility (EMBL) N/A Ni-NTA Agarose Qiagen Cat#: 30210 cOmplete Protease Inhibitor Cocktail Roche Cat#: 11697498001 DAPI Roche Cat#: 10236276001 Imidazole Roth Cat#: 3899.4 Gelatine Sigma Cat#: G1890 2-Mercaptoethanol Sigma Cat#: M6250 Isopropyl b-D-1thiogalactopyranoside (IPTG) Sigma Cat#: I5502 Adonitol, 99% Thermo Fisher Scientific Cat#: L03253 Methoxylamine hydrochloride, 25-30% aq. soln.



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